Shigella induces epigenetic reprogramming of zebrafish neutrophils
Gomes, MC; Brokatzky, D
; Bielecka, MK; Wardle, FC and Mostowy, S
(2023).
Shigella induces epigenetic reprogramming of zebrafish neutrophils.
[Dataset].
NCBI Gene Expression Omnibus.
https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE217063
; Bielecka, MK; Wardle, FC and Mostowy, S
(2023).
Shigella induces epigenetic reprogramming of zebrafish neutrophils.
[Dataset].
NCBI Gene Expression Omnibus.
https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE217063
The ChIP-seq data of neutrophils 48 hours after Shigella training for: "Shigella induces epigenetic reprogramming of zebrafish neutrophils".
Keywords
Mitochondria; Neutrophils; Shigella flexneri; Trained Immunity; Zebrafish| Item Type | Dataset |
|---|---|
| Resource Type |
Resource Type Resource Description Dataset Quantitative |
| Description of data capture | Trained immunity is a long-term memory of innate immune cells, generating an improved response upon re-infection. Shigella is an important human pathogen and inflammatory paradigm for which there is no effective vaccine. Using zebrafish larvae we demonstrate that after Shigella priming neutrophils are more efficient at bacterial clearance. We observe that Shigella-induced protection is non-specific and long-lasting, and is unlike training by BCG and β-glucan. Analysis of histone ChIP-seq on primed neutrophils revealed that Shigella training deposits the active H3K4me3 mark on promoter regions of 1612 genes, significantly changing the epigenetic landscape of neutrophils towards enhanced microbial recognition and mitochondrial ROS production. Finally, we demonstrate that mitochondrial ROS plays a key role in enhanced antimicrobial activity of trained neutrophils. It is envisioned that signals and mechanisms we discover here can be used in other vertebrates, including humans, to suggest new therapeutic strategies involving neutrophils to control bacterial infection. |
| Capture method | Unknown |
| Date | 13 September 2023 |
| Language(s) of written materials | English |
| Creator(s) |
Gomes, MC; Brokatzky, D; Bielecka, MK; Wardle, FC and Mostowy, S
|
| LSHTM Faculty/Department | Faculty of Infectious and Tropical Diseases > Department of Infection Biology |
| Participating Institutions | London School of Hygiene & Tropical Medicine, London, United Kingdom; King's College London, London, United Kingdom |
| Funders |
Project Funder Grant Number Funder URI UNSPECIFIED BB/R016283/ http://dx.doi.org/10.13039/501100000268 UNSPECIFIED UNSPECIFIED http://dx.doi.org/10.13039/501100001255 |
| Date Deposited | 16 Feb 2024 09:47 |
| Last Modified | 28 Aug 2025 12:51 |
| Publisher | NCBI Gene Expression Omnibus |
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